postgraduate student 204 «Technologies of production and processing of livestock products» specialization, Junior researcher of the Laboratory of Genetics
Institute of Pig Breeding and Agricultural Production NAAS
The purpose of this work was to determine the origin of pigs (Large White × Landrace) × Maxgro of the final hybrid using mitochondrial DNA markers. As a genetic material used bristles from the auricle of commercial pigs (large white × landrace) × Maxgro. DNA secretion was carried out according to the method of Korinnyi S.M., etc.2005, using Chelex ion exchange resin – 100. For the analysis of the mitochondrial genome, the method of polymorphism of the lengths of restriction fragments amplified in PCR was used (Polymerase chain reaction-restriction fragment of polymorphism – PCR-RFLP). The D-loop of the mitochondrial genome of a pig of 428 pairs is subject to analysis of nucleotide with Tasl recognition sites in positions 15558, 15580, 15616, 15714, 15758). Comparison of inherited on the maternal line restrict fragments in the breed allowed get reliable information about the origin of the experimentally studied sample of pigs of the final hybrid (n=15) from RPE "Globinsky Pig Farm", Globyno, town Poltava region, Ukraine allowed getting reliable information of their origin. Laboratory research was carried out based at the Institute of Pig Breeding and Agricultural Production NAAS in the Laboratory of Genetics. By analyzing nucleotide sequences, one monomorphic site was identified and experimentally investigated in position 15558 p.n. and three polymorphic sites in positions 15580, 15616, 15714, 15758 p.n. for endonuclease TasI (↓AATT). To systematize combinations of mitochondrial DNA restriction fragments, three breed-specific mitochondrial haplotypes were identified in an experimentally studied sample of hybrid pigs (n=15). With its use, mitochondrial haplotypes of pigs of the final hybrid were determined: 4 animals with haplotype C, 6 with haplotype N, and 5 with haplotype O. According to many authors pieces of research, these haplotypes characterize different breeds, namely C – Landrace, N – Large White (Asian type) and O – Landrace. The obtained data on the origin of animals of the final hybrid suggests that two-breed sows were the result of direct (Large White × Landrace) and reciprocal crossing (Landrace × Large White). Genetic examination in the establishment of maternal breeds of hybrid pigs with the help of markers of the mitochondrial genome and the search for a polymorphic area of the X and Y-chromosome to determine the ancestral line has become an urgent issue in continuing our research in modern pig breeding technologies of commercial lines. The work was done with the support of the National Academy of Agrarian Sciences of Ukraine 31.01.00.07. F. “Investigate the pleiotropic effect gens that the SNP use in marker-associated pig breeding”. DR № 0121U109838.
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